These should be provided
before July 1, 2008.
Applications from students from under-represented groups are strongly encouraged.
Although the integration of modern molecular assays into marine microbial ecology has provided an enormous amount of information about the diversity, distribution, and abundance of microbial populations, most often the information cannot be linked back to a particular cell or population. Light and epi-fluorescent microscopy are excellent platforms for enumerating and identifying planktonic (in particular, phytoplanktonic) populations. The individuals most and best trained in cell identification by microscopy and basic cell counting techniques are typically in the more senior positions and are reaching the point of retirement. It seems only necessary to provide a platform for the dissemination of the knowledge and invaluable skill sets of our most experienced microscopists to the next generation of scientists.
Here, the National Science Foundation-funded Center for Microbial Oceanography: Research and Education (C-MORE) is proud to offer a 5-day workshop on phytoplankton and bacterioplankton identification led by experienced senior and junior marine microbial ecologists at the Hawai‘i Institute of Marine Biology (HIMB), Coconut Island, Hawai‘i.
Our microscopy workshop will be a practical guide for identifying the major phytoplanktonic groups of Kaneohe Bay, HI using light and blue/green epifluorescence microscopy. In addition, proper procedures (statistical considerations) for phytoplankton and bacterioplankton cell counting and various staining techniques (e.g. DAPI, FISH) will be reviewed and processed by participants. Participants will also collect and process their own field samples (whole water and net tows) using a small boat operation. Students will receive a “cookbook” of microscopy protocols, procedures, and cultivation strategies.
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